All Datasets

Dataset

CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

  • Dataset ID:DS-10442

Release Date: 2024-10-15

Last Modified: 2024-10-15

key visualization for CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

Dataset Overview

Raw movie stacks, tilt series, alignments and cryo-eletron tomograms of synthetically reduced genome cells. JCVI-Syn3A has 493 genes (about half of the genes of the WT Mycoplasma Mycoides Capri GM12). Samples were prepared by plunge-freezing via Leica GP2. Contained data was collected on a Krios G4 using a Falcon 4i detector and SelctrisX energy filter. Tomograms were reconstructed using AreTomo3 v1.0.23. The deposition also includes locations of cytosolic ribosomes identified using slabpick and membrane segmenations created using membrain-seg.

Publications

Not Submitted

Related Databases

Not Submitted

Runs

262 of 262 Runs

Run Name

Tilt Series Quality Score

Annotated Objects

key visualization for 24jul17a_Position_7_5
24jul17a_Position_7_5

Run ID: RN-16391

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_35_3
24jul16a_Position_35_3

Run ID: RN-16318

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10_4
24aug01b_Position_10_4

Run ID: RN-16204

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_47_3
24jul17a_Position_47_3

Run ID: RN-16369

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_27_9
24jul17a_Position_27_9

Run ID: RN-16345

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_44_4
24jul24a_Position_44_4

Run ID: RN-16446

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_13
24jul24a_Position_13

Run ID: RN-16404

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_37_4
24jul17a_Position_37_4

Run ID: RN-16357

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_61
24jul17a_Position_61

Run ID: RN-16379

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_35
24jul17a_Position_35

Run ID: RN-16354

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_31
24jul24a_Position_31

Run ID: RN-16424

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_30
24jul16a_Position_30

Run ID: RN-16313

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_32
24jul24a_Position_32

Run ID: RN-16426

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_37_3
24jul17a_Position_37_3

Run ID: RN-16356

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_49_4
24jul24a_Position_49_4

Run ID: RN-16453

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_107
24jul17a_Position_107

Run ID: RN-16331

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_39_4
24jul24a_Position_39_4

Run ID: RN-16440

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_21_2
24aug01b_Position_21_2

Run ID: RN-16220

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_37_2
24jul24a_Position_37_2

Run ID: RN-16436

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul17a_Position_85_2
24jul17a_Position_85_2

Run ID: RN-16397

4 - Good
  • cytosolic ribosome
  • membrane
  • 1
  • 2
  • 3
  • 4
  • 5
  • 14